The DNA puff BhB10-1 gene is differentially expressed in various tissues of Bradysia hygida late larvae and constitutively transcribed in transgenic Drosophila

Brazilian Journal of Medical and Biological Research

N. Monesi¹  J.f. Sousa¹  M.l. Paçó-larson¹ 

[1] ,Universidade de São Paulo Faculdade de Medicina de Ribeirão Preto Departamento de Biologia Celular e Molecular e Bioagentes PatogênicosRibeirão Preto SP ,Brasil

关键词: DNA puff;    gene expression;    development;    ecdysone;    transgenic Drosophila;   

DOI  :  10.1590/S0100-879X2001000700003

来源: SciELO

【 摘 要 】

We extended the characterization of the DNA puff BhB10-1 gene of Bradysia hygida by showing that, although its mRNA is detected only at the end of the fourth larval instar, BhB10-1 expression is not restricted to the salivary gland, the tissue in which this gene is amplified. Different amounts of BhB10-1 mRNA were detected in other larval tissues such as gut, Malpighian tubules, fat body, brain and cuticle, suggesting that this gene is expressed differentially in the various tissues analyzed. Analysis of transgenic Drosophila carrying the BhB10-1 transcription unit and flanking sequences revealed that the tested fragment promotes transcription in a constitutive manner. We suggest that either cis-regulatory elements are missing in the transgene or factors that temporally regulate the BhB10-1 gene in B. hygida are not conserved in Drosophila.

【 授权许可】

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