Ecdysone Triggers the Expression of Golgi Genes in Drosophila Imaginal Discs via Broad-Complex

Developmental Biology

Jonathan C. Dunne¹  Vangelis Kondylis¹  Catherine Rabouille¹ 

[1] The Wellcome Trust Centre for Cell Biology, ICMB, The Michael Swann Building, University of Edinburgh, Mayfield Road, Edinburgh, EH9 3JR, United Kingdom

关键词: Golgi stacks;    Golgi proteins;    ecdysone;    Broad-complex;    gene expression;    in situ hybridisation;    FISH;    immunofluorescence;   

DOI  :  10.1006/dbio.2002.0632

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

OneofthemostsignificantmorphogeniceventsinthedevelopmentofDrosophilamelanogasteristheelongationofimaginaldiscsduringpupariumformation.WehaveshownthatthismacroscopiceventisaccompaniedbytheformationofGolgistacksfromsmallGolgilarvalclustersofvesiclesandtubulesthatarepresentpriortotheonsetofdiscelongation.Wehaveshownthattheflysteroidhormone20-hydroxyecdysonetriggersboththeelongationitselfandtheformationofGolgistacks(V.Kondylis,S.E.Goulding,J.C.Dunne,andC.Rabouille,2001,Mol.Biol.Cell,12,2308).UsingmRNAinsituhybridisation,weshowherethatecdysonetriggerstheupregulationofasubsetofgenesencodingGolgi-relatedproteins(suchasdnsf1,dsec23,dsed5,anddrab1)anddownregulatestheexpressionofothers(suchasdergic53,dβ'COP,anddrab6).WeshowthatthetranscriptionfactorBroad-complex,itselfan“early”ecdysonetarget,mediatesthisregulation.Andweshowthattheecdysone-independentupregulationofdnsf1anddsnappriortotheecdysonepeakleadstoaprecociousformationoflargeGolgistacks.Theecdysone-triggeredbiogenesisofGolgistacksattheonsetofimaginaldiscelongationofferstheexcitingpossibilityofadvancingourunderstandingoftherelationshipbetweengeneexpressionandorganellebiogenesis.

【 授权许可】

Unknown