Antibodies against the Plasmodium falciparum glutamate-rich protein from naturally exposed individuals living in a Brazilian malaria-endemic area can inhibit in vitro parasite growth

Memórias do Instituto Oswaldo Cruz

Lilian Rose Pratt-riccio¹  Cesare Bianco-junior¹  Paulo Renato Rivas Totino¹  Daiana De Souza Perce-da-silva¹  Luciene Aquino Silva¹  Evelyn Kety Pratt Riccio¹  Vítor Ennes-vidal¹  Ana Gisele Costa Neves-ferreira¹  Jonas Perales¹  Surza Lucia Gonçalves Da Rocha¹  Fabrício Dias-da-silva¹  Maria De Fátima Ferreira-da-cruz¹  Cláudio Tadeu Daniel-ribeiro¹  Joseli De Oliveira-ferreira²  Michael Theisen¹  Leonardo José De Moura Carvalho¹  Dalma Maria Banic¹ 

[1] ,Instituto Oswaldo Cruz-Fiocruz Laboratório de Imunoparasitologia Rio de Janeiro RJ ,Brasil

关键词: malaria;    Plasmodium falciparum;    GLURP;    antibodies;    biological activity;    growth inhibition;   

DOI  :  10.1590/S0074-02762011000900005

来源: SciELO

【 摘 要 】

The glutamate-rich protein (GLURP) is an exoantigen expressed in all stages of the Plasmodium falciparum life cycle in humans. Anti-GLURP antibodies can inhibit parasite growth in the presence of monocytes via antibody-dependent cellular inhibition (ADCI), and a major parasite-inhibitory region has been found in the N-terminal R0 region of the protein. Herein, we describe the antiplasmodial activity of anti-GLURP antibodies present in the sera from individuals naturally exposed to malaria in a Brazilian malaria-endemic area. The anti-R0 antibodies showed a potent inhibitory effect on the growth of P. falciparum in vitro, both in the presence (ADCI) and absence (GI) of monocytes. The inhibitory effect on parasite growth was comparable to the effect of IgGs purified from pooled sera from hyperimmune African individuals. Interestingly, in the ADCI test, higher levels of tumour necrosis factor alpha (TNF-α) were observed in the supernatant from cultures with higher parasitemias. Our data suggest that the antibody response induced by GLURP-R0 in naturally exposed individuals may have an important role in controlling parasitemia because these antibodies are able to inhibit the in vitro growth of P. falciparum with or without the cooperation from monocytes. Our results also indicate that TNF-α may not be relevant for the inhibitory effect on P. falciparum in vitro growth.

【 授权许可】

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