Methicillin-resistant Staphylococcus aureus in human milk

Memórias do Instituto Oswaldo Cruz

Fr Novak²  Jag Almeida¹  Mb Warnken¹  Bt Ferreira-carvalho²  An Hagler² 

[1] ,Universidade Federal do Rio de JaneiroRio de Janeiro RJ ,Brasil

关键词: human milk methicillin;    resistant;    Staphylococcus aureus;    enterotoxin;    Brazil;   

DOI  :  10.1590/S0074-02762000000100003

来源: SciELO

【 摘 要 】

We collected and analyzed 500 samples of human milk, from five Brazilian cities (100 from each) to detect methicillin-resistant strains of Staphylococcus aureus (MRSA) producing enterotoxins. We found 57 strains of MRSA, and the mecA gene, responsible for resistance, was detected in all of them using a specific molecular probe. We examined 40 strains for the presence of four enterotoxins, after selecting a subset that included all strains from each region, except for the largest sample, from which 10 were randomly selected. Among these two presented enterotoxin B, and growth in human colostrum and trypicase soy broth. After 5 h of incubation at 37°C, population sizes were already higher than 9.4 x 105 UFC/ml and enterotoxin was released into culture medium and colostrum. Our results stress the importance of hygiene, sanitary measures, and appropriate preservation conditions to avoid the proliferation of S. aureus in human milk.

【 授权许可】

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