Epitope Analysis of Staphylococcal Enterotoxin A Using Different Synthetic Peptides

Journal of Veterinary Medical Science

Akio NAKANE²  Dong-Liang HU¹  Ayako IMAI¹  Katsuhiko ONO¹  Shunji SUGII³  Sanae SASAKI²  Kunihiro SHINAGAWA¹ 

[1] Department of Veterinary Medicine, Faculty of Agriculture, Iwate University;Department of Bacteriology, Hirosaki University School of Medicine;Department of Veterinary Science, College of Agriculture, Osaka Prefectural University

关键词: enterotoxin;    epitope;    peptide;    Staphylococcus aureus;   

DOI  :  10.1292/jvms.60.993

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(5)The antigenic determinants (or epitopes) of staphylococcal enterotoxin A (SEA) were analyzed using synthetic peptides and rabbit antibodies to their corresponding peptides. Of 12 different synthetic peptides tested, peptides A-1 (corresponding to the amino acid sequence 1-20), A-5 (81-100), and A-8 (141-160) were reactive with anti-SEA antibodies. However, all synthetic peptides were found to elicit antibodies reactive with native SEA molecule. These findings suggest that native SEA molecule contains at least 3 different antigenic determinants.

【 授权许可】

Unknown