Immunogenicity of multiple antigen peptides containing Plasmodium vivax CS epitopes in BALB/c mice

Memórias do Instituto Oswaldo Cruz

Myriam A. Herrera²  Cecilia De Plata²  Jhon Mario González¹  Giampietro Corradin¹  Socrates Herrera² 

[1] ,Universidad del Valle School of Health Cali,Colombia

关键词: malaria;    Plasmodium vivax;    vaccines;    synthetic peptides;   

DOI  :  10.1590/S0074-02761994000600017

来源: SciELO

【 摘 要 】

Multiple antigen peptide systems (MAPs) allow the incorporation of various epitopes in to a single synthetic peptide immunogen. We have characterized the immune response of BALB/c mice to a series of MAPs assembled with different B and T cell epitopes derived from the Plasmodium vivax circumsporozoite (CS) protein. A B-cell epitope from the central repeat domain and two T-cell epitopes from the amino and carboxyl flanking regions were used to assembled eight different MAPs. An additional universal T cell epitope (ptt-30) from tetanus toxin protein was included. Immunogenicity in terms of antibody responses and in vitro T lymphocyte proliferation was evaluated. MAPs containing B and T cell epitopes induced high titers of anti-peptides antibodies, which recognized the native protein on sporozoites as determined by IFAT. The antibody specificity was also determined by a competitive inhibition assay with different MAPs. A MAP containing the B cell epitope (p11) and the universal epitope ptt-30 together with another composed of p11 and the promiscuous T cell epitope (p25) proved to be the most immunogenic. The strong antibody response and specificity for the cognate protein indicates that further studies designed to assess the potential of these proteins as human malaria vaccine candidates are warranted.

【 授权许可】

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