Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells

Anais da Academia Brasileira de Ciências

Betânia S. Monteiro²  Napoleão M. Argôlo-neto²  Nance B. Nardi¹  Pedro C. Chagastelles¹  Pablo H. Carvalho²  Laila P. Bonfá²  Richard R. Filgueiras¹  Amanda S. Reis²  Ricardo J. Del Carlo² 

[1] ,Universidade Federal de Viçosa Departamento de Veterinária Viçosa MG ,Brasil

关键词: Bone therapy;    Mesenchymal stem cell;    Autologous graft;    Mice;    Calvaria;    terapia óssea;    células-tronco mesenquimais;    enxerto autólogo;    camundongo;    calvária;   

DOI  :  10.1590/S0001-37652012000300026

来源: SciELO

【 摘 要 】

Mesenchymal stem cells (MSC) are present in specialized niches in perivascular regions of adult tissues and are able to differentiate into various cell types, such as those committed to repairing. Bone marrow derived MSC from eight young mice C57BL/ 6 gfp+ were expanded in culture for repairing critical defects in calvarial bone produced in twenty-four young isogenic adult C57BL/6 mice. The animals were subjected to a cranial defect of 6.0mm diameter and divided into two equal experimental groups. Control group did not receive any treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL into the defects. The group treated with MSC showed increased angiogenesis and amount of new bone deposited on the defect limits than that observed in the control group. The results demonstrated that transplantation of bone marrow-derived MSC of C57BL/6 gfp+ mice to bone critical defects produced in mice calvarial contributes positively to the bone repair process. MSC presets ability to influence the correct functioning of osteoblasts, increases the amount of mobilized cells for the repairing process, speeds up growth, and increases deposition of bone matrix.

【 授权许可】

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