Integrated experimental platforms to study blast injuries: a bottom-up approach

18th APS-SCCM; 24th AIRAPT

Bo, C.^1,2 ; Williams, A.^3 ; Rankin, S.^1,4 ; Proud, W.G.^1,2 ; Brown, K.A.^1,5,6

Royal British Legion Centre for Blast Injury Studies, Imperial College London, London, United Kingdom^1

Institute of Shock Physics, Imperial College London, London, United Kingdom^2

Department of Veterinary Medicine, Cambridge University, Cambridge, United Kingdom^3

National Heart and Lung Institute, Imperial College London, London, United Kingdom^4

Cavendish Laboratory, Cambridge University, Cambridge, United Kingdom^5

Department of Chemistry, University of Texas, Austin, United States^6

关键词: Biological properties;    Experimental models;    Experimental platform;    Integrated research;    Mesenchymal stem cell;    Quasi-static experiments;    Soft biological tissue;    Split Hopkinson pressure bars;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/500/10/102001/pdf
DOI  :  10.1088/1742-6596/500/10/102001

来源: IOP

【 摘 要 】

We are developing experimental models of blast injury using data from live biological samples. An integrated research strategy is followed to study material and biological properties of cells, tissues and organs, that are subjected to dynamic and static pressures, relevant to those of battlefield blast. We have developed a confined Split Hopkinson Pressure Bar (SHPB) system, which allows cells, either in suspension or as a monolayer, to be subjected to compression waves with pressures on the order of a few MPa and durations of hundreds of microseconds. The chamber design enables recovery of biological samples for cellular and molecular analysis. The SHPB platform, coupled with Quasi-Static experiments, is used to determine stress-strain curves of soft biological tissues under compression at low, medium and high strain rates. Tissue samples are examined, using histological techniques, to study macro- and microscopic changes induced by compression waves. In addition, a shock tube enables application of single or multiple air blasts with pressures on the order of kPa and a few milliseconds duration; this platform was used for initial studies on mesenchymal stem cells responses to blast pressures.