KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil

Brazilian Journal of Microbiology

Camila Arguelo Biberg¹  Ana Claudia Souza Rodrigues¹  Sidiane Ferreira Do Carmo¹  Claudia Elizabeth Volpe Chaves¹  Ana Cristina Gales¹  Marilene Rodrigues Chang¹ 

关键词: Klebsiella pneumoniae;    carbapenems;    drug-resistant bacteria;   

DOI  :  10.1590/S1517-838246246220140174

来源: SciELO

【 摘 要 】

The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the blaKPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009.

【 授权许可】

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