KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil

Revista de microbiologia

Rodrigues, Ana Claudia Souza¹  Biberg, Camila Arguelo²  Carmo, Sidiane Ferreira do³ 

[1] Hospital Regional de Mato Grosso do Sul, Campo Grande, Brasil;Universidade Federal de Mato Grosso do Sul, Campo Grande, Brasil;Universidade Federal de São Paulo, São Paulo, Brasil

关键词: Klebsiella pneumoniae;    carbapenems;    drug-resistant bacteria.;   

DOI  :  10.1590/S1517-838246246220140174

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the blaKPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009.

【 授权许可】

CC BY-NC