Comparison of an in-house and a commercial enzyme-linked immunosorbent assay (ELISA) for diagnosis of paratuberculosis

Brazilian Journal of Microbiology

Carla Dray Marassi²  Janete Da Silva Gonzaga²  Paula Ristow¹  Rachel Ferreira¹  Leila Souza Fonseca¹  Walter Oelemann²  Walter Lilenbaum¹ 

[1] ,Universidade Federal do Rio de Janeiro Departamento de Imunologia Rio de Janeiro,Brazil

关键词: Cattle;    Mycobacterium avium subsp. paratuberculosis;    serology;    Bovinos;    Mycobacterium avium subsp. paratuberculosis;    sorologia;   

DOI  :  10.1590/S1517-83822007000100002

来源: SciELO

【 摘 要 】

An in-house PPA-ELISA was compared to a commercial ELISA using a panel of 108 serum samples. In relation to commercial test, in-house assay presented 100% of sensitivity and 83.5% of specificity and a high concordance (kappa> 0.5), which demonstrates that the in-house assay is a valuable tool for use in developing countries.

【 授权许可】

CC BY-NC   
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