Reversion by calcium of a yeast-like development to the original filamentous form, of the 10V10 5-fluorocytosine-sensitive mutant of Aspergillus niger

Brazilian Journal of Microbiology

Rosangela De Carvalho Goulart²  José Moacir Marin¹ 

[1] ,Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Jaboticabal SP ,Brasil

关键词: Aspergillus niger;    5-fluorocytosine;    yeast-like;    calcium;    acid production;    dimorphism;    Aspergillus niger;    5-fluorocitosina;    leveduriforme;    cálcio;    produção de ácido;    dimorfismo;   

DOI  :  10.1590/S1517-83822005000300018

来源: SciELO

【 摘 要 】

Some filamentous fungi present the phenomenon of dimorphism, their morphological structure alterations being capable of inducing metabolism changes. The Aspergillus niger strain 10v10, a producer of citric acid, was submitted to the mutagenic action of ultraviolet irradiation which respectively selects mutants sensitive or resistant to the antifungi agent 5 fluorocytosine (5-FC). 5-FC sensitive mutants presented a morphological alteration to a yeast-like form. The effects of pH changes, addition of salts (KH2PO4, NH4NO3, MgSO4 and MgCl2), the presence of osmotic stabilizers, as well as of calcium chloride, on morphological reversal and acid production were studied. Morphological reversal to the filamentous form was observed only in the presence of CaCl2 (500mM) for the mutants strains 1 and 2, while the acid production occurred in both, yeast-like and filamentous forms.

【 授权许可】

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