Selection of 5-fluorocytosine-resistant mutants from an Aspergillus niger citric acid-producing strain

Brazilian Journal of Microbiology

Ana Paula De F. Conte¹  José M. Marin² 

[1] ,Faculdade de Ciências Farmacêuticas de Ribeirão PretoSão Paulo SP ,Brasil

关键词: Aspergillus niger;    citric acid;    5-fluorocytosine;    Aspergillus niger;    ácido cítrico;    5-fluorocitosina;   

DOI  :  10.1590/S1517-83822003000100001

来源: SciELO

【 摘 要 】

Mutants of Aspergillus niger N402, induced by UV mutagenesis, were selected and tested for resistance or sensitivity to 5-fluorocytosine. Some mutants showed increased citric acid production, which did not correlate with the intracellular amount of protein or ammonium ion. The resistance to 5-fluorocytosine proved to be a rational approach for isolation of new mutants with improved production of citric acid. The best mutant (FR13) accumulated double the amount of citric acid produced by the parental strain.

【 授权许可】

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