Isolation, properties and kinetics of growth of a thermophilic Bacillus

Brazilian Journal of Microbiology

Adriane Nunes De Souza¹  Meire Lelis Leal Martins¹ 

[1] ,Universidade Estadual do Norte Fluminense CCTA Laboratório de Tecnologia de Alimentos RJ ,Brasil

关键词: Thermophilic microorganism;    Bacillus;    isolation;    Microrganismo termófilo;    Bacillus;    Isolamento;   

DOI  :  10.1590/S1517-83822001000400003

来源: SciELO

【 摘 要 】

A strictly aerobic, thermophilic, spore-forming bacteria was isolated from a soil sample collected in Campos dos Goytacazes City, Rio de Janeiro, Brazil. The cells of this organism were Gram-positive, catalase positive, actively motile, 1.2 µm wide and 5.3 µm long. Growth occurred at pH values ranging from 6.5 to 9.0, and optimum growth occurred at about pH 7.0. The optimum temperature for growth was around 55ºC, and the upper temperature limit for growth was around 70ºC. Yeast extract stimulated growth on glucose and was obligately required at supraoptimal temperatures (65ºC). The results of 16S rRNA sequence comparisons indicated that the isolate was closely related to Bacillus caldoxylolyticus and Bacillus sp strain AK1, and these three organisms exhibited levels of ribossomal DNA sequence homology of 94%.

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