Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene

Genetics and Molecular Biology

Mariza M. Coêlho²  Monica S. Ferreira-nozawa¹  Sérgio R. Nozawa¹  André L.w. Santos² 

[1] ,Universidade Nilton Lins Laboratório de Biotecnologia Vegetal Manaus AM ,Brazil

关键词: isolation;    sequencing;    Bacillus;    Pantoea;   

DOI  :  10.1590/S1415-47572011005000045

来源: SciELO

【 摘 要 】

Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species.

【 授权许可】

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