Extraction of PCR-amplifiable genomic DNA from Bacillus anthracisspores

Torok, Tamas

Lawrence Berkeley National Laboratory

关键词: Federal Bureau Of Investigation;    Dna;    59;    Bacillus;    B. Anthracis Endospore Disruption Dna Extraction;   

DOI  :  10.2172/917812
RP-ID  :  LBNL--52707
RP-ID  :  DE-AC02-05CH11231
RP-ID  :  917812

美国|英语

来源: UNT Digital Library

【 摘 要 】

Bacterial endospore disruption and nucleic acid extractionresulting in DNA of PCR-amplifiable quality and quantity are not trivial.Responding to the needs of the Hazardous Materials Response Unit (HMRU),Laboratory Division, Federal Bureau of Investigation, protocols weredeveloped to close these gaps. Effectiveness and reproducibility of thetechniques were validated with laboratory grown pure spores of Bacillusanthracis and its close phylogenetic neighbors, and with spiked soils anddamaged samples.