Molecular analysis of endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.)

Brazilian Journal of Microbiology

José Edson Fontes Figueiredo²  Eliane Aparecida Gomes¹  Claudia Teixeira Guimarães¹  Ubiraci Gomes De Paula Lana¹  Marta Aparecida Teixeira²  Guilherme Vitor Corrêa Lima¹  Wellington Bressan¹ 

[1] ,Embrapa Milho e Sorgo Laboratório de Bioquímica Molecular Sete Lagoas MG ,Brasil

关键词: endophytic bacteria;    Bacillus;    sweet corn;    SDS-PAGE;    rDNA sequencing;    Bactéria endofítica;    Bacillus;    milho doce;    SDS-PAGE;    seqüenciamento de rDNA;   

DOI  :  10.1590/S1517-83822009000300014

来源: SciELO

【 摘 要 】

Endophytic bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of wholecell protein extract of fortytwo isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosomal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing, Bacillus subitilis and B. pumilus were the most frequenty species (15 and 12 isolates, respectively) followed by B. licheniformes (7 isolates), B. cereus (5 isolates) and B. amiloliquefascens (3 isolates). According to present results, SDS-PAGE technique could be used as a fast and cheap first tool for identifying interspecific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intraspecific variation among isolates with similar protein profile as well as for taxonomic studies.

【 授权许可】

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