Sperm Quality and Testicular Histomorphometry of Wistar Rats Supplemented with Extract and Fractions of Fruit of Tribulus terrestris L.

Brazilian Archives of Biology and Technology

Nelma Neylanne Pinho Muniz Oliveira¹  Matheus Augusto Rodrigues Félix¹  Tauany Costa Silva Pereira¹  Luiz Gustavo Pessoa Rocha¹  José Rafael Miranda¹  Márcio Gilberto Zangeronimo¹  José Eduardo Brasil Pereira Pinto¹  Suzan Kelly Vilela Bertolucci¹  Raimundo Vicente De Sousa¹ 

关键词: Vegetal extract;    Spermatogenesis;    Leydig cells;   

DOI  :  10.1590/S1516-89132015060278

来源: SciELO

【 摘 要 】

ABSTRACT The aim of this study was to assess the sperm quality and testicular histomorphometry of Wistar rats supplemented with extract and fractions of fruits of Tribulus terrestris L. The ethanolic extract was obtained by dynamic maceration of spray-dried fruit. This extract was fractionated by liquid-liquid partition, using increasing polarity solvents. Twenty male rats were separated in four groups, with five rats in each group. The control was supplemented with distilled water, while the others were daily given the ethanolic extract, hexanic or aqueous fraction soluble in methanol in a dose of 42 mg.kg-1.day-1 for 70 days. Sperm was obtained from the right epididymal tail for the analysis of motility, count, morphology and viability. The testicular weight of groups supplemented with ethanolic extract and aqueous fraction soluble in methanol was higher when compared to the control. The gonadosomatic index increased in the group supplemented with ethanolic extract. The nuclear, cytoplasmic and individual volume of Leydig cells increased in supplementation with hexanic and aqueous fractions soluble in methanol. It was concluded that the extract influenced the spermatogenesis, while hexanic and aqueous fractions soluble in methanol promoted the changes in the intertubular compartment. Therefore, Tribulus terrestris did not improve the sperm quality of the rats.

【 授权许可】

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