Chemical Shift Images of Organelles in Leydig cells of Mice Testes

11th International Conference on X-ray Microscopy

Ejima, T.^1 ; Neichi, Y.^1 ; Yanagihara, M.^1 ; Kado, M.^2 ; Ishino, M.^2 ; Yasuda, K.^3 ; Tamotsu, S.^3

Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1, Katahira, Aoba-ku, 980-8577, Sendai, Japan^1

Kansai Photon Science Institution, Japan Atomic Energy Agency, 8-1-7, Umemidai, Kizugawa, 619-0215, Kyoto, Japan^2

Research Group of Biological Sciences, Faculty Division of Natural Sciences, NaraWomen's University, Kitauoyahigashimachi, 630-8506, Nara, Japan^3

关键词: Absorbances;    Differential interference;    Image comparison;    Incident wavelength;    Leydig cells;    Peak structure;    Soft X-ray;    Transmission images;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/463/1/012047/pdf
DOI  :  10.1088/1742-6596/463/1/012047

来源: IOP

【 摘 要 】

Soft X-ray transmission images of Leydig cells of mice testes changing incident wavelength were observed with the use of a contact microscope. After normalization of transmission images, absorbance images were obtained and compared with a visible differential interference image. Some organelles were identified by the image comparison, and absorption spectra of the organelles were obtained from the absorbance images. The absorption spectra show that peak structures are different depending on the observed organelles. The structures and the positions of organelles were clearly identified at C-K absorption.