Methodological variations in the isolation of genomic DNA from Streptococcus bacteria

Brazilian Archives of Biology and Technology

Mônica Moreira²  Juliana Noschang²  Ivana Froede Neiva²  Yanê Carvalho¹  Llma Hiroko Higuti²  Vânia Aparecida Vicente² 

[1] ,Universidade Federal do Paraná Departamento de Patologia Básica Curitiba PR ,Brasil

关键词: Streptococcus;    DNA isolation;    CTAB;    ultrasound;   

DOI  :  10.1590/S1516-89132010000400013

来源: SciELO

【 摘 要 】

In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility.

【 授权许可】

CC BY-NC   
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