The isolation of high molecular mass DNA from the prochlorophyte Prochloron didemni

FEBS Letters

Larkum, Anthony W.D.¹  Lockhart, Peter J.¹  Howe, Christopher J.²  Beanland, Timothy J.² 

[1] Department of Biological Sciences, University of Sydney, Sydney, NSW 2006, Australia;Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England

关键词: Prochlorophyte;    Evolution;    DNA isolation;    Cloning;    (Prochloron didemni);   

DOI  :  10.1016/0014-5793(89)80446-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a protocol known to inactivate endogenous nucleases in other organisms we have been able to isolate high molecular mass DNA from Prochloron didemni. This DNA is shown to be suitable for restriction endonuclease analysis and the generation of genomic libraries in the λ cloning vector EMBL3.

【 授权许可】

Unknown