Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)

Acta Agronómica

Leterme, Pascal¹  Murgueitio, Enrique²  Muñoz Flórez, Jaime Eduardo¹  Cárdenas Henao, Heiber³  Posso Terranova, Andrés Mauricio¹ 

[1] Universidad Nacional de Colombia sede Palmira, Palmira,, Colombia;Fundación Centro para la Investigación en Sistemas Sostenibles de Producción Agropecuaria (CIPAV), Cali, Colombia;Universidad del Valle, Santiago de Cali, Colombia

关键词: DNA;    DNA isolation;    genetic markers;    germplasm banks;    nacedero;    Trichanthera gigantea;   

DOI  :  

学科分类：农业科学（综合）

来源: Universidad Nacional de Colombia * Facultad de Ciencias Agropecuarias Palmira

【 摘 要 】

To study the genetic diversity of the national collection of Trichanthera gigantea (Humb. & Bonpl.) Nees from the Foundation Center for Research in Sustainable Systems for Agricultural Production (CIPAV), five primers RAMs were used on 38 accessions from 11 departments of Colombia and two states of Venezuela. A pattern of 71 bands was obtained and used to estimate genetic diversity parameters. A specific protocol for DNA isolation was adapted and a DNA bank was established at the National University of Colombia, Palmira campus for further investigations. The results showed heterogeneity and high genetic diversity in these accessions. No relationship was found among molecular group analysis and the geographical origin zones.

【 授权许可】

CC BY-NC-SA