Dose assessment by quantification of chromosome aberrations and micronuclei in peripheral blood lymphocytes from patients exposed to gamma radiation

Genetics and Molecular Biology

Isvânia Silva-barbosa²  Símey Pereira-magnata²  Ademir Amaral²  Graça Sotero¹  Homero Cavalcanti Melo¹ 

[1] ,Universidade Federal de Pernambuco Departamento de Energia Nuclear Grupo de Estudos em Radioproteção e RadioecologiaRecife PE ,Brazil

关键词: chromosome aberrations;    micronuclei;    biodosimetry;    partial-body irradiation;    lymphocyte;   

DOI  :  10.1590/S1415-47572005000300021

来源: SciELO

【 摘 要 】

Scoring of unstable chromosome aberrations (dicentrics, rings and fragments) and micronuclei in circulating lymphocytes are the most extensively studied biological means for estimating individual exposure to ionizing radiation (IR), which can be used as complementary methods to physical dosimetry or when the latter cannot be performed. In this work, the quantification of the frequencies of chromosome aberrations and micronuclei were carried out based on cytogenetic analyses of peripheral blood samples from 5 patients with cervical uterine cancer following radiotherapy in order to evaluate the absorbed dose as a result of partial-body exposure to 60Co source. Blood samples were collected from each patient in three phases of the treatment: before irradiation, 24 h after receiving 0.08 Gy and 1.8 Gy, respectively. The results presented in this report emphasize biological dosimetry, employing the quantification of chromosome aberrations and micronuclei in lymphocytes from peripheral blood, as an important methodology of dose assessment for either whole or partial-body exposure to IR.

【 授权许可】

CC BY   
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