Heritable Genetic Changes in Cells Recovered From Irradiated 3D Tissue Constructs

Michael Cornforth

关键词: CHROMOSOMES;    DNA;    GENETICS;    INTERSTITIALS;    MICROARRAY TECHNOLOGY;    RADIATIONS;    IRRADIATION Ionizing radiation;    chromosome aberrations;    low dose;    LET;   

DOI  :  10.2172/1037011
RP-ID  :  DOEER64347
PID  :  OSTI ID: 1037011
Others  :  TRN: US201208%%873

美国|英语

来源: SciTech Connect

【 摘 要 】

Combining contemporary cytogenetic methods with DNA CGH microarray technology and chromosome flow-sorting increases substantially the ability to resolve exchange breakpoints associated with interstitial deletions and translocations, allowing the consequences of radiation damage to be directly measured at low doses, while also providing valuable insights into molecular mechanisms of misrepair processes that, in turn, identify appropriate biophysical models of risk at low doses. Specific aims apply to cells recovered from 3D tissue constructs of human skin and, for the purpose of comparison, the same cells irradiated in traditional 2D cultures. The project includes research complementary to NASA/HRP space radiation project.