Micropropagation, seed propagation and germplasm bank of Mandevilla velutina (Mart.) Woodson

Scientia Agricola

Ronaldo Biondo²  Ana Valéria Souza²  Bianca Waléria Bertoni²  Andreimar Martins Soares¹  Suzelei Castro França²  Ana Maria Soares Pereira² 

[1] ,UNAERP Unidade de Biotecnologia Ribeirão Preto SP ,Brasil

关键词: Apocynaceae;    Cerrado;    medicinal plant;    Apocynaceae;    Cerrado;    planta medicinal;   

DOI  :  10.1590/S0103-90162007000300008

来源: SciELO

【 摘 要 】

Mandevilla velutina (Mart.) Woodson (Apocynaceae) is a medicinal plant species with antivenom properties, native from Brazilian Savanna regions (Cerrado), which due to overexploitation and habitat deforestation is in danger of extinction. As an initiative for conserving this endangered but economically important plant species, a micropropagation protocol was developed and genotypes were stored in the Germplasm Bank "Cerrado In vitro". For the in vitro propagation of M. velutina, nodal segments were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations of BA, Zeatin, 2ip, DTT and TDZ. Best multiplication ratio was achieved when to the medium 0.44 µM BA, ranging 1: 6.7, were added. Plantlets cultured on MS/2 medium supplemented with 26.85 µM NAA rooted successfully (50.5%). Although rooted and un-rooted plantlets acclimatized to soil conditions, great losses were observed within un-rooted plantlets, while the rooted presented 100 % survival. It was possible to maintain 43% of the M. velutina germplasm under healthy conditions for six months, with no subcultures, using the MS medium supplemented with 2% sucrose, 13.8 mM spermidine, 2% sorbitol and 2% dextrose.

【 授权许可】

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