Journal of the Brazilian Chemical Society | |
A chemometric model applied to fatty acid determination in blood | |
Thiago I. B. Lopes2  Casey A. Rimland2  Sabrina Nagassaki1  Bruno Geloneze1  Anita J. Marsaioli2  | |
[1] ,Universidade Estadual de Campinas Institute of Chemistry Campinas SP ,Brazil | |
关键词: fatty acid methyl esters; principal component analysis; selected ion monitoring; | |
DOI : 10.5935/0103-5053.20130201 | |
来源: SciELO | |
【 摘 要 】
Changes in fatty acid profiles have been associated with several pathophysiological processes. Gas chromatography-mass spectrometry (GC-MS) data monitoring of selected ions was used with principal components analysis (PCA), revealing a set of relevant ions for quantification and characterization of fatty acids. This protocol was successfully applied to the analyses of fatty acids in different human blood lipids, allowing the quantification of several fatty acids and revealing their unsaturation numbers. Moreover the presences of contaminants, artifacts and co-eluitions in the chromatogram were also revealed without any additional analyses. Thus, fatty acid constituents of triglycerides, phospholipids and esters of cholesterol present in plasma and erythrocyte membranes were accurately determined, with repeatability, low limits of determination and of quantification.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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