A chemometric model applied to fatty acid determination in blood

Journal of the Brazilian Chemical Society

Geloneze, Bruno¹  Marsaioli, Anita J.¹  Universidade Estadual de Campinas, Campinas, Brazil¹  Lopes, Thiago I. B.¹  Nagassaki, Sabrina¹  Rimland, Casey A.¹ 

关键词: fatty acid methyl esters;    principal component analysis;    selected ion monitoring;   

DOI  :  10.5935/0103-5053.20130201

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Changes in fatty acid profiles have been associated with several pathophysiological processes. Gas chromatography-mass spectrometry (GC-MS) data monitoring of selected ions was used with principal components analysis (PCA), revealing a set of relevant ions for quantification and characterization of fatty acids. This protocol was successfully applied to the analyses of fatty acids in different human blood lipids, allowing the quantification of several fatty acids and revealing their unsaturation numbers. Moreover the presences of contaminants, artifacts and co-eluitions in the chromatogram were also revealed without any additional analyses. Thus, fatty acid constituents of triglycerides, phospholipids and esters of cholesterol present in plasma and erythrocyte membranes were accurately determined, with repeatability, low limits of determination and of quantification.

【 授权许可】

Unknown