Preferential utilization of endogenous arachidonate by cyclo‐oxygenase in incubations of human platelets

FEBS Letters

Caruso, D.¹  Paoletti, R.¹  Galli, G¹  Sautebin, L.¹ 

[1] Institute of Pharmacology and Pharmacognosy, University of Milan, Via A. del Sarto 21, Milan, Italy

关键词: TXB2;    12-HETE;    Arachidonic acid;    Cyclo-oxygenase;    Lipoxygenase;    Human platelets;    TXA2;    thromboxane A2;    TXB2;    thromboxane B2;    12-HETE;    12-hydroxy-5;    8;    10;    14-eicosatetraenoic acid;    12-HPETE;    12-hydroperoxy-5;    8;    10;    14-eicosatetraenoic acid;    HHT;    12-hydroxy-5;    8;    10-heptadecatrienoic;    PGs;    prostaglandins;    PRP;    platelet-rich plasma;    SIM;    selected ion monitoring;    RIA;    radioimmunoassay;    TLC;    thin-layer chromatography;    RGLC;    radiogaschromatography;    BSTFA;    N;    O-bis-(trimethylsilyl)-trifluoroacetamide;   

DOI  :  10.1016/0014-5793(83)81140-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thromboxane B₂ (TXB₂) and 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE) formed from the endogenous and exogenous arachidonate during human platelet incubation, was evaluated by selected ion monitoring (SIM). TXB₂ formed from endogenous substrate accounted for about one third of the total, whereas the great part of 12-HETE derived from exogenous arachidonate. These data indicate that under the tested conditions the pool of arachidonate that acts as substrate for cyclo-oxygenase is different from the pool that acts as substrate for lipoxygenase and that the arachidonate released from phospholipids is preferentially utilized by cyclo-oxygenase.

【 授权许可】

Unknown