Induction of cyclo‐oxygenase by interleukin‐1 in rheumatoid synovial cells

FEBS Letters

Barrett, M.L.¹  O'Neill, L.A.J.¹  Lewis, G.P.¹ 

[1] Department of Pharmacology, Hunterian Institute, Royal College of Surgeons, London WC2A 3PN, England

关键词: Interleukin-1;    Prostaglandin E2;    Cyclo-oxygenase;    (Human rheumatoid synovial cell);    IL-1;    interleukin-1;    RASC;    rheumatoid adherent synovial cells;    PGE2;    prostaglandin E2;    DMEM;    Dulbecco's modified Eagle's medium;   

DOI  :  10.1016/0014-5793(87)81551-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ability of interleukin-1 (IL-1) to stimulate prostaglandin E₂ (PGE₂ production by human rheumatoid adherent synovial cells was found to be time-dependent and sensitive to protein synthesis inhibitors. Cells incubated with exogenous arachidonic acid (10 μM) showed no increase in PGE₂ production. However, with IL-1 (2.5 ) and exogenous arachidonic acid there was a marked increase, with levels reaching twice that for cells incubated with IL-1 alone. Aspirin pre-treatment studies and the use of [acetyl-¹⁴C]aspirin showed that IL-1 increased PGE₂ production through the induction of cyclo-oxygenase.

【 授权许可】

Unknown