Optimization of extraction and determination of chloramphenicol in livestock meat samples using aqueous two-phase system of n-propanol and potassium citrate coupled with HPLC

Journal of the Brazilian Chemical Society

Yanfang Li²  Juan Han²  Yun Wang²  Jingjing Ma¹  Yongsheng Yan²  Lihua Cao¹ 

[1] ,Jiangsu University School of Chemistry and Chemical Engineering Zhenjiang,China

关键词: HPLC;    aqueous two-phase system;    extraction;    chloramphenicol;    livestock meat;    ATPS;   

DOI  :  10.5935/0103-5053.20130088

来源: SciELO

【 摘 要 】

An aqueous two-phase system (ATPS) based on short chain alcohol and salt was the method for preconcentration, separation and analysis of chloramphenicol (CAM), coupled with high performance liquid chromatography with ultraviolet-visible detector (HPLC-UV). In order to select the suitable ATPS for CAM extraction, different ATPSs were tested and n-propanol/potassium citrate ATPS was chosen for separating and concentrating CAM in the subsequent experiments. The influences of the salt concentration, pH and the volume of n-propanol on the extraction efficiency and partition coefficient of CAM were examined. Response surface methodology was employed to optimize the experimental conditions. Under the optimal conditions, this method has been applied to quantitative determination of CAM in livestock meat samples with limit of detection of 0.48 ng g-1 and limit of quantification of 1.6 ng g-1 with a recovery range of 92.39-104.12%. This ATPS used low cost of organic solvents and supplied a moderate and biocompatible environment, which is suitable for biomolecules.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License