Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)

Journal of the Brazilian Chemical Society

Túlio O. G. Costa²  Richardson A. Almeida²  Jorge T. Melo¹  Hector H. F. Koolen¹  Felipe M. A. Da Silva²  José Roberto S. A. Leite¹  Maura V. Prates¹  Carlos Bloch Jr.¹  Angelo C. Pinto¹ 

[1] ,Universidade Federal do Amazonas Departamento de Química Manaus AM ,Brazil

关键词: anionic peptide;    antimicrobial peptide;    Osteocephalus taurinus;    skin secretion;   

DOI  :  10.1590/S0103-50532012001200002

来源: SciELO

【 摘 要 】

By means of chromatographic techniques, a novel anionic peptide, named otacidin, was isolated from the skin secretions of tree Brazilian anurans Osteocephalus taurinus. The de novo sequencing of the amino acids was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS) analyses. The antimicrobial potential of this novel metabolite was evaluated against Escherichia coli and Pseudomonas aeruginosa, being observed minimum inhibitory concentration (MICs) of 37.4 and 78.4 mM, respectively. This work is just the second report of an antimicrobial anionic peptide from an amphibian.

【 授权许可】

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