Cinerascetins, New Peptides from Hypsiboas cinerascens: MALDI LIFT-TOF-MS/MS de novo Sequence and Imaging Analysis

Journal of the Brazilian Chemical Society

Richardson A. Almeida¹  Marcelo Gordo¹  Felipe M. A. Da Silva¹  Rafael C. De Araújo¹  Marcelo H. S. Ramada¹  Fernando Y. Abrão¹  Túlio O. G. Costa¹  Hector H. F. Koolen¹  Afonso D. L. De Souza¹  Carlos Bloch Jr.¹ 

关键词: anuran;    antimicrobial peptide;    Hypsiboas cinerascens;    skin secretion;   

DOI  :  10.5935/0103-5053.20150219

来源: SciELO

【 摘 要 】

The continuous search for antimicrobial candidates pushes the pursuit of compounds in the most diverse organisms. Amphibians are known as a prolific source of antibacterial peptides. Based on the rich biodiversity of the Amazon region the unexplored green-tree frog (Hypsiboas cinerascens) was studied for its skin secretion peptide content. Chromatographic separations and established tandem mass spectrometry (MS/MS) methods were used for sequencing the primary structures of the purified compounds. De novo sequencing lead to the identification of five new peptides related to hylaseptin P1, displaying an aminated C-terminal. Sequencing of the complementary deoxyribonucleic acid (cDNA) analysis allowed the disambiguation of isobaric amino-acids for C-01. Matrix assisted laser desorption ionization (MALDI) was carried out, demonstrating the in situ co-occurrence of the identified peptides in the dorsal skin. The major peptide C-01 was synthesized and assayed against a selection of microorganisms displaying minimal inhibitory concentrations (MICs) ranging from 4 to 16 µM.

【 授权许可】

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