Cinerascetins, New Peptides from Hypsiboas cinerascens: MALDI LIFT-TOF-MS/MS de novo Sequence and Imaging Analysis

Journal of the Brazilian Chemical Society

Souza, Afonso D. L. de¹  Koolen, Hector H. F.¹  Bloch Jr., Carlos¹  Gordo, Marcelo¹  Ramada, Marcelo H. S.¹  Universidade de Brasília, Brasília, Brazil¹  Universidade Federal do Amazonas, Manaus, Brazil¹  Costa, Túlio O. G.¹  Araújo, Rafael C. de¹  Universidade Federal de Goiás, Goiânia, Brazil¹  Abrão, Fernando Y.¹  Embrapa-Recursos Genéticos e Biotecnologia, Brasília, Brazil¹  Almeida, Richardson A.¹  Silva, Felipe M. A. da¹ 

关键词:  anuran;    antimicrobial peptide;    Hypsiboas cinerascens;    skin secretion;   

DOI  :  10.5935/0103-5053.20150219

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

The continuous search for antimicrobial candidates pushes the pursuit of compounds in the most diverse organisms. Amphibians are known as a prolific source of antibacterial peptides. Based on the rich biodiversity of the Amazon region the unexplored green-tree frog (Hypsiboas cinerascens) was studied for its skin secretion peptide content. Chromatographic separations and established tandem mass spectrometry (MS/MS) methods were used for sequencing the primary structures of the purified compounds. De novo sequencing lead to the identification of five new peptides related to hylaseptin P1, displaying an aminated C-terminal. Sequencing of the complementary deoxyribonucleic acid (cDNA) analysis allowed the disambiguation of isobaric amino-acids for C-01. Matrix assisted laser desorption ionization (MALDI) was carried out, demonstrating the in situ co-occurrence of the identified peptides in the dorsal skin. The major peptide C-01 was synthesized and assayed against a selection of microorganisms displaying minimal inhibitory concentrations (MICs) ranging from 4 to 16 µM.

【 授权许可】

Unknown