Isolation and amino acid sequencing by MALDI-TOF-MS/MS of a novel antimicrobial anionic peptide from the skin secretion of Osteocephalus taurinus (Anura, Hylidae)

Journal of the Brazilian Chemical Society

Melo, Jorge T.¹  Koolen, Hector H. F.¹  Bloch Jr., Carlos¹  Universidade Federal do Piauí, Parnaíba, Brazil¹  Universidade Federal do Amazonas, Manaus, Brazil¹  Costa, Túlio O. G.¹  Leite, José Roberto S. A.¹  Universidade Estadual de Campinas, Campinas, Brazil¹  Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil¹  Almeida, Richardson A.¹  Silva, Felipe M. A. da¹  EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, Brazil¹  Prates, Maura V.¹  Pinto, Angelo C.¹ 

关键词: anionic peptide;    antimicrobial peptide;    Osteocephalus taurinus;    skin secretion.;   

DOI  :  10.1590/S0103-50532012001200002

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

B> By means of chromatographic techniques, a novel anionic peptide, named otacidin, was isolated from the skin secretions of tree Brazilian anurans Osteocephalus taurinus. The de novo sequencing of the amino acids was performed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS) analyses. The antimicrobial potential of this novel metabolite was evaluated against Escherichia coli and Pseudomonas aeruginosa, being observed minimum inhibitory concentration (MICs) of 37.4 and 78.4 mM, respectively. This work is just the second report of an antimicrobial anionic peptide from an amphibian.

【 授权许可】

Unknown