Can Wistar rats be used as the normotensive controls for nerve morphometry investigations in spontaneously hypertensive rats (SHR)?

Acta Cirurgica Brasileira

Luciana Sayuri Sanada²  Marcelo Rodrigo Tavares¹  Milena Cardoso Maia Neubern¹  Helio Cesar Salgado¹  Valéria Paula Sassoli Fazan¹ 

[1] ,USP FMRP Department of Neuroscience and Behavioral NeurosciencesRibeirao Preto SP ,Brazil

关键词: Sural Nerve;    Rats;    Inbred SHR;    Rats;    Inbred WKY;    Nerve Fibers;    Rats;    Nervo Sural;    Ratos Endogâmicos SHR;    Ratos Endogâmicos WKY;    Fibras Nervosas;    Ratos;   

DOI  :  10.1590/S0102-86502011000600018

来源: SciELO

【 摘 要 】

PURPOSE: We compared the sural nerve morphology among Wistar (WR), Wistar-Kyoto (WKY) and Spontaneously hypertensive (SHR) rats, including the nerve fascicles and myelinated fibers morphometry. METHODS: Age matched (20 weeks) female WR (N=6), WKY (N=6) and SHR (N=7) had their right and left sural nerves removed, embedded in epoxy resin, and observed by light microscopy. Morphometric analysis was performed with the aid of computer software. RESULTS: Despite presenting the same age, WR were heavier than WKY and SHR, as were SHR compared to WKY. Systolic arterial pressure was higher in SHR compared to WR, but no differences between SHR and WKY or WR and WKY were observed. The sural nerves were morphometrically symmetric between proximal and distal segments on the same side and between sides in all strains with no differences in the myelinated fiber number. Schwann cell number and density were smaller in SHR and G ratio was larger in SHR, indicating that SHR have thinner myelinated fibers. CONCLUSION: Sural nerve morphology is similar between WKY and WR, allowing the use of WR as the SHR controls in morphological investigations involving peripheral neuropathies.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License