The effect of laser on remanescent liver tissue after 90% hepatectomy in rats

Acta Cirurgica Brasileira

Alexandre Ferreira Oliveira²  Tiago Castro E Silva¹  Ajith Kumar Sankarankutty¹  Eduardo Garcia Pacheco¹  Juliana Ferreira¹  Vanderlei Salvador Bagnato¹  Sergio Zucoloto¹  Orlando De Castro E Silva¹ 

[1] ,USP FMRP Departament of Surgery and Anatomy,Brazil

关键词: Liver Regeneration;    Acute Liver Failure;    Laser;    Partial Hepatectomy;    Regeneração Hepática;    Insuficiência Hepática Aguda;    Laser;    Hepatectomia parcial;   

DOI  :  10.1590/S0102-86502006000700007

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the effect of laser beam on remanescent liver after partial hepatectomy 90%. METHODS: Wistar rats, (N= 42), were divided into six groups with seven specimens each. The partial hepatectomy (HP) was performed in all animals through exeresis of approximately 90% of the liver parenchyma. The animals from groups HP and Laser application, HPL24, HPL48 and HPL72 undertook laser irradiation carried out through application (dose of 22.5 J/cm²) in five different sites in the remanescent liver. The rats were then sacrificed 24, 48 and 72hours after HP procedure, for the liver regeneration analysis,using the Proliferating Cell Nuclear Antigen (PCNA),and for dosage of serum aminotransferases. RESULTS: Were demonstrated an increase of the serum levels of alanine aminotransferase for the group of 24 hours and a decrease for the group of 72 hours exposed to laser. The index of marked cells had a considerable more improvement for the group of 72 hours exposed in laser compared to other groups. CONCLUSION: Laser did not cause hepatic injuries additional to the partial hepatectomy and perhaps led to a benefit by stimulating the proliferative activity.

【 授权许可】

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