Ochratoxin A determination in beer by immunoaffinity column clean-up and high-performance liquid chromatography

Food Science and Technology (Campinas)

Guilherme Prado²  Marize Silva Oliveira²  Eliana Pinheiro Carvalho¹  Luiz Carlos Oliveira Lima¹  Thais Veloso²  Leandro Augusto Ferreira Souza¹  Ana Cristina Ferreira Cardoso¹ 

[1] ,Fundação Ezequiel Dias Núcleo de Micologia e Micotoxinas Belo Horizonte MG

关键词: ochratoxin A;    beer;    immunoaffinity;    HPLC;    ocratoxina A;    cerveja;    imunoafinidade;    CLAE;   

DOI  :  10.1590/S0101-20612003000400011

来源: SciELO

【 摘 要 】

Analyses of ochratoxin A (OTA) in domestic and imported beers were perfomed by immunoaffinity column and high - perfomance liquid chromatography (HPLC) using a fluorescence detector. Recoveries of OTA from beer samples spiked at levels from 8.0 to 800pg/mL ranged from 81.2% to 95.0%, with coefficient of variation between 0% e 11.0%. Detection limit and quantification limit were 2.0pg/mL and 8.0pg/mL, respectively. Of the total of 26 samples produced in Brazil only 6 (23%), contained trace amounts of OTA. Of the 4 imported beers, in 2, Ireland and Germany, were detected OTA at levels of 25pg/mL and 82pg/mL, respectively.

【 授权许可】

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