Titanium Dioxide Nanoparticles (TiO2) Quenching Based Aptasensing Platform: Application to Ochratoxin A Detection

Toxins

Atul Sharma¹  Akhtar Hayat¹  Rupesh K. Mishra¹  Gaëlle Catanante¹  Sunil Bhand²  Jean Louis Marty¹ 

[1] BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France; E-Mails:;Biosensor Lab, Department of Chemistry, BITS, Pilani- K. K. Birla Goa Campus, Zuarinagar, Goa 403726, India; E-Mail:

关键词: titanium dioxide nanoparticles;    fluorescently labeled aptamer;    aptamer assay;    ochratoxin A;    beer;    quenching;   

DOI  :  10.3390/toxins7093771

来源: mdpi

【 摘 要 】

We demonstrate for the first time, the development of titanium dioxide nanoparticles (TiO₂) quenching based aptasensing platform for detection of target molecules. TiO₂ quench the fluorescence of FAM-labeled aptamer (fluorescein labeled aptamer) upon the non-covalent adsorption of fluorescent labeled aptamer on TiO₂ surface. When OTA interacts with the aptamer, it induced aptamer G-quadruplex complex formation, weakens the interaction between FAM-labeled aptamer and TiO₂, resulting in fluorescence recovery. As a proof of concept, an assay was employed for detection of Ochratoxin A (OTA). At optimized experimental condition, the obtained limit of detection (LOD) was 1.5 nM with a good linearity in the range 1.5 nM to 1.0 µM for OTA. The obtained results showed the high selectivity of assay towards OTA without interference to structurally similar analogue Ochratoxin B (OTB). The developed aptamer assay was evaluated for detection of OTA in beer sample and recoveries were recorded in the range from 94.30%–99.20%. Analytical figures of the merits of the developed aptasensing platform confirmed its applicability to real samples analysis. However, this is a generic aptasensing platform and can be extended for detection of other toxins or target analyte.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.