Detection of cytotoxic activity on Vero cells in clinical isolates of Serratia marcescens

Brazilian Journal of Medical and Biological Research

G.v. Carbonell²  A.f. Alfieri¹  A.a. Alfieri¹  M.c. Vidotto¹  C.e. Levy¹  A.l.c. Darini¹  R.m. Yanaguita² 

[1] ,Universidade de São Paulo

关键词: cytotoxin production;    nosocomial infections;    plasmids;    Serratia marcescens;    virulence factors;   

DOI  :  10.1590/S0100-879X1997001100005

来源: SciELO

【 摘 要 】

Cytotoxin production was studied in 60 Serratia marcescens strains isolated from hospitalized patients. Association of cytotoxic activity with serotype, source of isolation and presence of plasmids was also evaluated. Thirteen of the 60 S. marcescens strains produced a cytotoxic effect on Vero cells. These strains were isolated from distinct clinical sources and classified into seven different serotypes (O1:H7; O4:NM; O10:NT; O19:NM; O6,14:H4; O6,14:NM and O6,14:H1). No relationship was observed between cytotoxic activity and clinical source or serotypes of the strains. Plasmids from five cytotoxin-producing S. marcescens strains were transferred to E. coli K12/711. The transconjugants did not exhibit cytotoxicity, indicating that the cytotoxic effect is not plasmid-mediated among these strains. Although a cytotoxic activity was demonstrated in filtrates of some S. marcescens strains, further studies should be performed to assess the role of this toxin in pathogenesis

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