Determinação de bussulfano em plasma através de cromatografia líquida de alta eficiência com detector de arranjo de diodos e derivatização com dietilditiocarbamato de sódio

Química Nova

Charline Fernanda Backes²  Sandrine Comparsi Wagner²  Fernando Dal Pont Morisso²  Marina Venzon Antunes²  Rafael Linden²  Marcelo Capra¹ 

[1] ,Universidade Feevale Instituto de Ciências da Saúde Novo Hamburgo RS ,Brasil

关键词: busulfan;    HPLC;    diethyldithiocarbamate;   

DOI  :  10.1590/S0100-40422012000700031

来源: SciELO

【 摘 要 】

A high performance liquid chromatographic-diode array detection method for the determination of busulfan in plasma was developed and validated. Sample preparation consisted of protein precipitation followed by derivatization with sodium diethyldithiocarbamate and liquid-liquid extraction with methyl-tert-butyl ether. Chromatograms were monitored at 277 nm. Separation was carried out on a Lichrospher RP 18 column (5 µm, 250 x 4 mm). The mobile phase consisted of water and acetonitrile (20:80, v/v). The method presented adequate specificity, linearity, precision and accuracy and allowed reliable determination of busulfan in clinical plasma samples, being applied to three patients submitted to bone marrow transplantation.

【 授权许可】

CC BY-NC   
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