Determination of busulfan in plasma by high performance liquid chromatography with diode array detector and derivatization with sodium diethyldithiocarbamate

Quimica nova

Capra, Marcelo¹  Linden, Rafael²  Morisso, Fernando Dal Pont²  Wagner, Sandrine Comparsi²  Antunes, Marina Venzon²  Backes, Charline Fernanda² 

[1] Irmandade Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, Brasil;Universidade Feevale, Novo Hamburgo, Brasil

关键词: busulfan;    HPLC;    diethyldithiocarbamate.;   

DOI  :  10.1590/S0100-40422012000700031

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A high performance liquid chromatographic-diode array detection method for the determination of busulfan in plasma was developed and validated. Sample preparation consisted of protein precipitation followed by derivatization with sodium diethyldithiocarbamate and liquid-liquid extraction with methyl-tert-butyl ether. Chromatograms were monitored at 277 nm. Separation was carried out on a Lichrospher RP 18 column (5 µm, 250 x 4 mm). The mobile phase consisted of water and acetonitrile (20:80, v/v). The method presented adequate specificity, linearity, precision and accuracy and allowed reliable determination of busulfan in clinical plasma samples, being applied to three patients submitted to bone marrow transplantation.

【 授权许可】

CC BY