Aplicação da metodologia de superfície de resposta no estudo da produção e extração da poligalacturonase

Química Nova

Sharline Florentino De Melo Santos²  Gorete Ribeiro De Macedo²  Flávio Luiz Honorato Da Silva¹  Rosane Liege Alves De Souza¹  Gustavo Adolfo Saavedra Pinto¹ 

[1] ,Universidade Federal do Rio Grande do Norte Departamento de Engenharia Química Natal RN ,Brasil

关键词: solid-state fermentation;    cashew apple;    Aspergillus niger;   

DOI  :  10.1590/S0100-40422008000800010

来源: SciELO

【 摘 要 】

The aim of this work was to verify the effects of initial medium moisture content (U), addition of ammonium sulphate (N) and of potassium phosphate (P) in the production of the polygalacturonase through the solid-state fermentation, using cashew apple husk as substrate and Aspergillus niger CCT0916 as transformation agent. We also studied the best extraction conditions of the produced enzyme. The best condition of production was with U of 40%, 1% of N and 0% of P being reached an activity of the poligalacturonase of 10.1 U/g. The best extraction condition is an agitation system with a time of 100 min and a solvent-fermented medium volume ratio of 5 mL/g.

【 授权许可】

CC BY-NC   
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