Application of response surface methodology to study the production and the extraction of polygalacturonase

Química Nova

Embrapa Agroindústria Tropical¹  Pinto, Gustavo Adolfo Saavedra¹  Santos, Sharline Florentino de Melo¹  Universidade Federal do Rio Grande do Norte, Natal, Brasil¹  Souza, Rosane Liege Alves de¹  Macedo, Gorete Ribeiro de¹  Universidade Federal de Campina Grande, Campina Grande, Brasil¹  Silva, Flávio Luiz Honorato da¹ 

关键词: solid-state fermentation;    cashew apple;    Aspergillus niger.;   

DOI  :  10.1590/S0100-40422008000800010

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The aim of this work was to verify the effects of initial medium moisture content (U), addition of ammonium sulphate (N) and of potassium phosphate (P) in the production of the polygalacturonase through the solid-state fermentation, using cashew apple husk as substrate and Aspergillus niger CCT0916 as transformation agent. We also studied the best extraction conditions of the produced enzyme. The best condition of production was with U of 40%, 1% of N and 0% of P being reached an activity of the poligalacturonase of 10.1 U/g. The best extraction condition is an agitation system with a time of 100 min and a solvent-fermented medium volume ratio of 5 mL/g.

【 授权许可】

Unknown