Efeito da composição do solvente sobre a estabilidade de proteínas em soluções aquosas

Química Nova

Lúbia Cristina Fonseca¹  Natássia Caroline Resende Corrêa¹  Mario Da Silva Garrote-filho¹  Cleine Chagas Da Cunha¹  Nilson Penha-silva¹ 

关键词: chaotropics;    protein stability;    osmolytes;   

DOI  :  10.1590/S0100-40422006000300024

来源: SciELO

【 摘 要 】

A protein presents a native (N) macro state, which is functionally active, in equilibrium with the denatured (D) macro state, which is devoid of biological activity. An ensemble of microstates forms each macrostate. The denatured state comprises a greater ensemble of microstates than the native macrostate. The N-D equilibrium can be affected by several factors, that comprise the purity of the water, temperature, pH and solute concentration. This work discusses the influence of osmolytes and chaotropics on the N-D equilibrium in aqueous solutions.

【 授权许可】

CC BY-NC   
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