Effects of the solvent composition on the stability of proteins in aqueous solutions

Química Nova

Cunha, Cleine Chagas da¹  Garrote-Filho, Mario da Silva¹  Corrêa, Natássia Caroline Resende¹  Penha-Silva, Nilson¹  Fonseca, Lúbia Cristina¹ 

关键词: chaotropics;    protein stability;    osmolytes.;   

DOI  :  10.1590/S0100-40422006000300024

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A protein presents a native (N) macro state, which is functionally active, in equilibrium with the denatured (D) macro state, which is devoid of biological activity. An ensemble of microstates forms each macrostate. The denatured state comprises a greater ensemble of microstates than the native macrostate. The N-D equilibrium can be affected by several factors, that comprise the purity of the water, temperature, pH and solute concentration. This work discusses the influence of osmolytes and chaotropics on the N-D equilibrium in aqueous solutions.

【 授权许可】

Unknown