Effects of the solvent composition on the stability of proteins in aqueous solutions

Quimica nova

关键词: chaotropics;    protein stability;    osmolytes.;   

DOI  :  10.1590/S0100-40422006000300024

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A protein presentsa native (N) macro state, which is functionally active, in equilibrium withthe denatured (D) macro state, which is devoid of biological activity. An ensembleof microstates forms each macrostate. The denatured state comprises a greaterensemble of microstates than the native macrostate. The N-D equilibrium canbe affected by several factors, that comprise the purity of the water, temperature,pH and solute concentration. This work discusses the influence of osmolytesand chaotropics on the N-D equilibrium in aqueous solutions.

【 授权许可】

CC BY