Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene

Pesquisa Agropecuária Brasileira

Rogério Abdallah Curi²  Luis Artur Loyola Chardulo¹  Antônio Carlos Silveira²  Henrique Nunes De Oliveira² 

[1] ,Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Departamento de Melhoramento e Nutrição AnimalBotucatu SP ,Brazil

关键词: calpastatin gene;    meat texture;    PCR-RFLP;    SNP;    gene da calpastatina;    textura da carne;    PCR-RFLP;    SNP;   

DOI  :  10.1590/S0100-204X2008000500014

来源: SciELO

【 摘 要 】

The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure.

【 授权许可】

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