Identification of Pit-1 Gen Using PCR-RFLP of Padjadjaran Sheep and Evaluate of Growth Rate

International Ruminant Seminar: "Eco-friendly livestock production for sustainable agriculture"

农业科学;生态环境科学

Prajoga, S.B.K.^1 ; Andriani, L.^1 ; Subhandiana, H.^2

Faculty of Animal Husbandry, Padjadjaran University, Jl. Raya Bandung - Sumedang Km 21, Jatinangor Bandung, Indonesia^1

Research Assistant of Animal Breeding and Biometric Laboratory, Animal Husbandry Faculty, Padjadjaran University, Bandung, Indonesia^2

关键词: Birth weight;    Body weight;    Correlation value;    Electrophoretic migration;    PCR-RFLP;    Restriction enzymes;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/119/1/012030/pdf
DOI  :  10.1088/1755-1315/119/1/012030

来源: IOP

【 摘 要 】

The objectives of this research were to evaluate variation of Pit-1 gen of Padjadjaran Sheep and evaluate of their growth rate. The data comprised of 15 lambs female and 15 lambs male records of 0 - 12 mouths old lambs. Variation of Padjadjaran Sheep PIT-1 gen was analyzed using PCR-RFLP and used primer by 5'-GAGGGATAATTACAAATGGTCC-3' and 5'-TGTTAACAGCTGTGGGACACAC-3', length fragment analyse using HinfI restriction enzyme. Presence or absence of deference bands in a PCR fragment of the result can be distinguished by differences in the electrophoretic migration of the fragment. The result showed that the variation Pit1 gene showed that there was in the position 345 bp, 137 bp and 115 bp. In this experiment the difference migration did occur in all samples (monomorphic), that was amplificated with reverse and forward primer. The average male birth weight (BW), weaning weight (WN) and Yearling Weight (YW) was 2.29 ±0.42kg; 8.96 ±1,89 kg and 30.12 ±5,65 kg. The average female birth weight (BW), weaning weight (WN) and Yearling Weight (YW) was 2.33 ±0.49 kg; 8.23 ±1.99 kg and 26.11 ±5.50 kg. Correlation value between age and body weight was 0,99 for female and 0,97 for male. The highest body weight gain per month was 2.85 kg for female at the age of six months and 3.4 kg for male at the age of one year. The best equition of growth rate was= 1,862 + 0,127X10,076X2