Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients

Revista da Sociedade Brasileira de Medicina Tropical

Ronaldo Luis Thomasini²  Juliana De Moraes Martins¹  Daniela Corte Parola¹  Sandra Helena Alves Bonon¹  Ilka De Fátima Santana Ferreira Boin¹  Luis Sérgio Leonardi¹  Marília Leonardi¹  Sandra Cecília Botelho Costa¹ 

[1] ,State University of Campinas Department of Pharmacology Campinas SP

关键词: Human herpesvirus-7;    Healthy individuals;    Nested-polymerase chain reaction;    Liver transplantation;    Herpesvirus humano-7;    Indivíduos sadios;    Nested-reação em cadeia da polimerase;    Transplante hepático;   

DOI  :  10.1590/S0037-86822008000600002

来源: SciELO

【 摘 要 】

Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host. The significance of viral DNA detected in leukocytes by PCR is unclear and cross-reaction in serological tests may occur. This study aimed to evaluate nested-PCR to detect human herpesvirus-7 active infection in liver transplant recipients compared to healthy individuals. human herpesvirus-7 nested-PCR was performed on leukocytes and sera of 53 healthy volunteers and sera of 29 liver transplant recipients. In healthy volunteers, human herpesvirus-7 was detected in 28.3% of leukocytes and 0% of serum. human herpesvirus-7 was detected in sera of 48.2% of the liver transplant recipients. Nested-PCR on DNA extracted from leukocytes detected latent infection and the study suggests that nested-PCR performed on serum could be useful to detect human herpesvirus-7 active infection in liver transplant recipients.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License