Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients

Revista da Sociedade Brasileira de Medicina Tropical

Thomasini, Ronaldo Luis¹  Leonardi, Marília¹  Martins, Juliana de Moraes¹  Bonon, Sandra Helena Alves¹  Hermínio Ometto Foundation, Araras¹  Leonardi, Luis Sérgio¹  Costa, Sandra Cecília Botelho¹  State University of Campinas, Campinas¹  Parola, Daniela Corte¹  Boin, Ilka de Fátima Santana Ferreira¹ 

关键词: Human herpesvirus-7;    Healthy individuals;    Nested-polymerase chain reaction;    Liver transplantation;   

DOI  :  10.1590/S0037-86822008000600002

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host. The significance of viral DNA detected in leukocytes by PCR is unclear and cross-reaction in serological tests may occur. This study aimed to evaluate nested-PCR to detect human herpesvirus-7 active infection in liver transplant recipients compared to healthy individuals. human herpesvirus-7 nested-PCR was performed on leukocytes and sera of 53 healthy volunteers and sera of 29 liver transplant recipients. In healthy volunteers, human herpesvirus-7 was detected in 28.3% of leukocytes and 0% of serum. human herpesvirus-7 was detected in sera of 48.2% of the liver transplant recipients. Nested-PCR on DNA extracted from leukocytes detected latent infection and the study suggests that nested-PCR performed on serum could be useful to detect human herpesvirus-7 active infection in liver transplant recipients.

【 授权许可】

Unknown